How is embryo freezing done?

How is an embryo frozen?
the main goal of embryo freezing is to hold the embryo for later use. the biggest trouble is the water inside the cells. whilst water freezes, crystals can shape. This growth can burst the cellular, inflicting it to die.

To save you this going on, the water within the embryo's cells is replaced with a protective substance called a cryoprotectant. The embryos are left to incubate in increasing ranges of cryoprotectant before they are frozen.

once most of the water has been eliminated, the embryo is cooled to its maintenance state via one in all two methods of embryo freezing:

sluggish freezing: This includes defensive the embryos from harm in sealed tubes after which slowly lowering the temperature inside the tubes. This prevents the embryo cells from growing older and becoming broken. Embryos can remaining tons longer of their frozen state than of their clean state. however, sluggish freezing is time-eating, and it requires highly-priced machinery.
Vitrification: on this procedure, the cryoprotected embryos are frozen so speedy that the water molecules in the embryos do now not have time to shape ice crystals. This helps to shield the embryos and to increase their survival price at some stage in thawing.
After freezing, the embryos are stored in liquid nitrogen until they're wished for destiny use.

success charges of thawing frozen embryos
The manner of thawing an embryo is fantastically a hit. a few studies has indicated that embryos frozen via vitrification have a better risk of survival, both at the freezing level and at some point of thawing.